OVOZOA
ISSN 2302-6464
Vol. 2 / No. 2 / Published : 2013-10
Order : 10, and page :74 - 81
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Original Article :
Effect of infusum salam leaves (eugenia polyantha) testis spermatogenic cells amount of white rats (rattus norvegicus) induced alloxan
Author :
- Gahastanira*1
- Wurlina *2
- Abdul Samik *3
- Budiarto *4
- Mahasiswa Fakultas Kedokteran Hewan Unair
- Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Unair
- Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Unair
- Departemen Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Unair
Abstract :
This research was aimed to know the effect of infusum salam leaves (Eugenia polyantha) on the appearance of testicular spermatogenic cells white rats (Rattus norvegicus) with diabetes mellitus. A number of 25 white rats (Rattus norvegicus) strain Wistar were divided into 5 groups. The pancreas of 4 groups was damaged using a doses of alloxan 200 mg/kg intraperitoneally. After state hyperglycemia condition, 3 groups was treatment with a dose therapy of infusum salam leaves (P1) 412.5 mg/150gbb, treatment (P2) 825 mg/150gbb and treatment (P3) 1237.5 mg/150gbb dose, one group as a control negative as the normal group and one group as a control positive was induced with alloxan but without infusum salam leaves treatment. The treatment was for 48 days, the mice were killed and were taken the testes organs to made a histopathology preparations using Hematoxylin-eosin staining (HE). The result of the study showed that infusum salam leaves (Eugenia polyantha) giving on treatment groups led to an increase in the number of spermatogenic cells (p <0.05) at all doses compared to positive controls. Treatment (P3) 1237.5 mg/150gbb dose can increase the amount of the highest spermatogenic cells.
Keyword :
diabetes mellitus, alloxan, infusum salam leaves, spermatogenic cells,
References :
Nugraheni, T., P. A. Okid dan T. Widiyani. ,(2005) Pengaruh Vitamin C terhadap Perbaikan Spermatogenesis dan Kualitas Spermatozoa Mencit (Mus musculus L.) Setelah Pemberian Ekstrak Tembakau (Nicotiana tabacum L.). Jakarta. : Biofarmasi. 1 (1): 13-19.
Nijveldt, R. T., N. Van., D. E. C. V. Hoorn, P.G. Boelens., K. V. Norren and P.A.M. V. Leeuwen. ,(2001) Flavonoids: A review of probable mechanism of action and potential applications. USA : Am. J. Clin. Nutr. 74: 418-425.
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