UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 1 / No. 1 / Published : 2012-10

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Original Article :

Effect of storage time of ram’s semen in several types of diluters on ram’s spermatozoa capacitation and acrosome reaction

Author :

  1. Suherni Susilowati*1
  2. Ainin Salasa*2
  3. Agus Sunarso*3
  1. Departemen Reproduksi Fakultas Kedokteran Hewan Unair
  2. Mahasiswa Fakultas Kedokteran Hewan Unair
  3. Departemen Parasitologi Fakultas Kedokteran Hewan Unair

Abstract :

Abstract   The aim of this research was to study the effect of storage time of ram’s semen in several types of diluters on the percentage of capacitation and acrosome reaction. Semen was collected six times using artificial vagina from the same ram, and then evaluated macroscopically and microscopically. Semen was divided into three groups which were diluted 5 times in Egg Yolk-Citrate (P1), Skim Milk-Egg Yolk (P2),  and Extract Banana-Citrate (P3). Motility and live percentages of ram’s spermatozoa were monitored everyday. Evaluation of capacitation and acrosome reaction percentages was conducted everyday at the same time after storage at 5°C (for motility percentages over 40%). The results showed that the degradation of capacitation and acrosome reaction percentage in P1, P2 and P3 was relatively stable during storage time, but according to the diluter, capacitation and acrosome reaction percentages of P1, P2 and P3 were not significantly different (P>0,05). The conclusion of this experiment was that storage time influenced capacitation and acrosome reaction of ram’s spermatozoa, but the type of diluter used in this experiment had no significant effect to the capacitation and acrosome reaction of ram’s spermatozoa.  

Keyword :

Egg Yolk, Citrate, Banana, capacitation, acrosome reaction,


References :

Aboagla, EM-E and T. Terada,,(2004) Effects of Egg Yolk during Defreezing Step of Cryopreservation on the Viability of Goat Spermatozoa. Theriogenology 62: 1160-1172. USA : Theriogenology





Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2012-10
  1. Influence Of Egg Yolk Skim Dan Egg Yolk Tris Extenders To Post Thawing Motility And Viability Merino Sheep Semen
  2. Motility And Viability Ram’s Spermatozoa On Various Silver Ion (ag+) Concentration In Egg Yolk Citrate Coconut Water Assortment Extender
  3. Effect Of Storage Time Of Ram’s Semen In Several Types Of Diluters On Ram’s Spermatozoa Capacitation And Acrosome Reaction
  4. Motility, Live Percentage And Completeness Of Post Thawing Thick Tail Sheep Membrane Spermatozoa In Three Variance Diluter

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