OVOZOA
ISSN 2302-6464
Vol. 7 / No. 1 / Published : 2018-04
Order : 3, and page :10 - 15
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Original Article :
Pengaruh penambahan l-arginin pada media pengencer skim-kuning telur terhadap membran plasma dan tudung akrosom spermatozoa domba merino post thawing
Author :
- Abidatul Maghfiroh*1
- Indah Norma T., drh., M.Si.*2
- Sunaryo Hadi W., drh., M.P.*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
This research was determined membrane integrity and acrosom cap Merino sheep post thawing after adding L-Arginine with different concentrations in extender skim milk and egg yolk to increased frozen semen quality. This research used fresh samples of Merino sheep’s semen collected by using artificial vagina, then where devided into 4 treatments and 6 replications. The experimental design that used was Completely Randomized Design. Analysis of the data using Analysis of Variance (ANOVA) one way then proceed to the Duncan to determine significant differences between treatments.The first treatment P0 no L-Arginine added as control. P1 was treated with 0,004 M L-Arginine. P2 was treated with 0,006 M L-Arginine. P3 was treated with 0,008 M L-Arginine. The result showed that determine significant different between treatments. The post thawing membrane integrity’s result was (P0) 25,50a± 2,73, (P1) 29,67b± 3,01, (P2) 33,00bc± 3,57, and (P3) 35,83c± 4,02. The post thawing acrosom cap’s result was (P0) 31,00a ± 1,78, (P1) 35,67b ± 3,26, (P2) 40,50c ± 2,42, and (P3) 42,33c ± 2,58. The optimum concentration added L-Arginine to increased membrane integrity and acrosom cap spermatozoa in this research was 0,006 M.
Keyword :
Merino sheep, spermatozoa, spermatozoa, L-Arginine, membrane integrity, acrosome cap,
References :
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