UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 4 / No. 2 / Published : 2015-10

Order : 8, and page :115 - 119

Related with : Scholar   Yahoo!   Bing

Original Article :

Pengukuran kadar protein spesifik fertility associated antigen (faa) vesikula seminalis sapi brangus menggunakan nano drop

Author :

  1. Nala Ni’amal Aza*1
  2. Anwar Ma’ruf*2
  3. Tri Wahyu Suprayogi*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Fertility Associated Atigen (FAA) was a protein which had a serves as a facilitator in the capacitation of spermatozoa during ejaculation. FAA presence in the plasma membrane of spermatozoa and plasma seminalis may increase fertility of the bulls. The purpose of this study was to calculate the protein content of the preparation of Fertility Associated Antigen (FAA) in the bull seminal vesicles (seminal plasma source). This study uses the seminal vesicles of nine male muzzle cow (S-1, S-2, S-3, S-4, S-5, S-6, S-7, S-8, S-9). Glands obtained from slaughterhouses, extracted using a muller mortar and mixed with PBS. Samples are identified using the technique of SDS-PAGE and Western blot, after the test using monoclonal antibody anti FAA of nine samples of only three samples that showed positive FAA is the sample S-1, S-3 and S-4. All three samples were then performed to obtain isolates FAA electroelution. Results FAA isolates of S-1 as much as 182 µl, S-3 as much 1200 µl and S-4 as much 1400 µl. Isolates were then done tera protein using Nano Drop, and the result is that the sample S-1 as much 0.260 ug/ml, sample S-3 as much as 0.164 ug/ml, and the samples S-4 as much 0.242 ug/ml. So it can be concluded on the seminal vesicles of male muzzle cow still contains a bit of the FAA, and the seminal vesicles which have isolates with high volume results do not necessarily have a high protein as well.

Keyword :

Seminal Vesicle, FAA, Elektroelusi, Nano Drop,


References :

Andrabi, S.M.H.,(2009) Review Article. Factor Affecting the Quality of Cryopreserved Buffallo (Bubalus Bubalis) Bull Spermatozoa. 44: 552-569. : Reproduction Domestic Animal.

Bellin, M.E., J.N. Oyarzo, H. E. Hawkins, H. Zhang, R. G. Smith, D. W. Forrest, and L. R. Sprott.,(1998) Fertility-associated Antigen on Bull Sperm Indicates Fertility Potential. 76:2032-2039. : Journal of Animal Science

Mc Cauley, T. C., G. R. Dawson, J. N. Oyarzo, J. McVicker, S. F. Marks, and R. L. Ax.,(2004) Development and validation of a lateral-flow cassette for fertility diagnosis in bulls. 10: 35-40. : IVD Technology

Noyes, J. H., K. Bruce, Johnson, D. Larry, Bryant, L. Scott, Findholt, J. W. Thomas.,(2011) Effects of Bull Age on Conception Dates and Pregnancy Rates of Cow Elk. 60(3): 508-517 : J Wildl Manage

Therien, I., R. Moreau, and P. Manjunath.,(1998) Major proteins of bovine seminal plasma and high-density lipoprotein induce cholesterol efflux from epididymal sperm. 59:



Archive Article

Cover Media Content

Volume : 4 / No. : 2 / Pub. : 2015-10
  1. Respon Injeksi Hormon Gonadotropin Dan Prostaglandin F2α Terhadap Angka Kebuntingan Dan Jumlah Anak Sekelahiran (litter Size) Mencit (mus Musculus)
  2. Hasil Sinronisasi Birahi Dan Kebuntingan Dengan Menggunakan Prostaglandin Pgf2 Alfa Dan Pmsg Pada Ibm Ternak Sapi Di Desa Kebontunggul, Kecamatan Gondang, Kabupaten Mojokerto
  3. Pengaruh Suhu Pada Proses Thawing Semen Beku Kambing Peranakan Etawa (pe) Yang Menggunakan Pengencer Andromed Terhadap Kualitas Spermatozoa
  4. Penampilan Reproduksi Kambing Peranakan Ettawa (pe) Di Wilayah Kecamatan Senduro, Kabupaten Lumajang, Jawa Timur
  5. Pengaruh Penambahan Propolis Dalam Pengencer Susu Skim Terhadap Viabilitas Dan Motilitas Spermatozoa Domba
  6. Pengaruh Penambahan Propolis Sebagai Antimikroba Dalam Pengencer Kuning Telur Sitrat Terhadap Viabilitas Dan Motilitas Spermatozoa Domba
  7. Pengaruh Waktu Thawing Semen Beku Kambing Peranakan Etawa Dalam Pengencer Andromed® Terhadap Motilitas, Viabilitas Dan Membran Plasma Spermatozoa.
  8. Pengukuran Kadar Protein Spesifik Fertility Associated Antigen (faa) Vesikula Seminalis Sapi Brangus Menggunakan Nano Drop
  9. Profil Progesteron Setelah Pemberian Prostaglandin F2α Pada Kuda Yang Mengalami Kista Korpus Luteum
  10. Identifikasi Kualitas Semen Dan Morfometri Spermatozoa Kambing Marica Sebagai Dasar Pembuatan Semen Beku
  11. Identifikasi Kejadian Hipofungsi Ovarium, Korpus Luteum Persisten Dan Kawin Berulang Pada Sapi Potong Daerah Dataran Tinggi Dan Dataran Rendah Kabupaten Nganjuk
  12. Efisiensi Reproduksi Dan Status Fertilitas Pada Akseptor Ib Di Kecamatan Sarirejo Kabupaten Lamongan Jawa Timur Pada Tahun 2012
  13. Pengaruh Waktu Pencabutan Sponge Intravaginal Progesterone (spinpro) Terhadap Timbulnya Birahi Dan Kebuntingan Kambing Peranakan Ettawa
  14. Hubungan Paritas Dengan Efisiensi Reproduksi Sapi Perah Peranakan Frisian Holstein (pfh) Di Koperasi Agroniaga (kan) Jabung Kabupaten Malang

Information detail_jurnal.php/OVOZOA