UNIVERSITAS AIRLANGGA



Detail Article

Planta Husada

ISSN 2338-7130

Vol. 2 / No. 1 / Published : 2014-04

Order : 5, and page :20 - 26

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Original Article :

Pinostrobin isolated from kaempferia pandurata roxb induced apoptosis in t47d human beast cancer cell line

Author :

  1. Sukardiman*1
  2. Aty Widyawaruyanti*2
  3. Retno Widyowati*3
  4. Sismindari*4
  5. Noor Cholies Zaini *5
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Dosen Fakultas Farmasi
  4. Universitas Gajah Mada
  5. Dosen Fakultas Farmasi

Abstract :

Pinostrobin isolated from Kaempferia pandurata Roxb. which belongs to the family of Zingiberaceae, was observed to induce apoptosis in T47D human breast cancer cell line. Pinostrobin at 10, 50, 100 mg/ml for 24, 48 and 72 h  induced DNA fragmentation and  increased the percentage of apoptotic cell after acridine orange – ethidium bromide staining. Our result demonstrated that pinostrobin can induce apoptosis in in T47D human breast cancer cell line in a time and concentration dependent manner by increase expression of p53, bax, caspase-3 and decreased expression of bcl-2 by immunohistochemical analysis.  

Keyword :

Pinostrobin, Kaempferia pandurata Roxb, T47D cell, Apoptosis, p53,


References :

Sukardiman,(2003) Aktivitas Antikanker Senyawa Pinostrobin dari Temu kunci (Kaempferia pandurata) terhadap Kultur Sel Mieloma Surabaya : Media Kedokteran Hewan

Sukardiman, Darwanto A, Tanjung M., Darmadi O,(2000) Cytotoxic Mechanism of Flavonoid from Temu Kunci (Kaempferia pandurata) in Cells Culture of Human Mammary Carcinoma - : Clinical Hemorheology and Microcirculation

Pommier Y,(1993) DNA topoisomerase I and IICancer Chemotheraphy: Update and Prospective - : Cancer Chemotheraphy. Pharmacology

Bail L, Aubourg L, Habrioux G,(2000) Effect of pinostrobin on estrogen metabolism and estrogen reseptor transactivivation - : Cancer Lett

Hsiang YH,(1989) Arrest of Replication Fork by Drug-stabilized Topoisomerase-DNA Cleaveable Complexes as a Mechanism of Cell Killing by Canpthothecin - : Cancer Research





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2014-04
  1. Eluent Effect On Sinensetin In Orthosiphon Stamineus Benth. Leaves Extract
  2. Ethyl Acetate Fraction Of Andrographis Paniculata Nees Increases Cytotoxic Effect Of Vincristine On Human Cancer Cell Lines
  3. Antiangiogenesis From Pericarp Of Mangosteen On T47d Breast Cancer
  4. Ethyl Acetate Fraction Of Johar Leaves (cassia Siamea Lamk) On Plasmodium Falciparum Strain G2300 (chloroquin Resistence) In Vitro As An Antimalaria Activity
  5. Pinostrobin Isolated From Kaempferia Pandurata Roxb Induced Apoptosis In T47d Human Beast Cancer Cell Line

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