UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 9 / No. 1 / Published : 2016-02

Order : 5, and page :23 - 28

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Original Article :

Supplementation of insulin transferrin selenium on in vitro bovine oocyte against to intracelular calcium expression

Author :

  1. Siti Eliana Rochmi*1
  2. Widjiati*2
  3. Hardijanto*3
  4. Wurlina*4
  1. Fakultas Vokasi Universitas Airlangga
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturationin molecular process with the increase of intracellular calsium expression as the promoting re-meiosis in oocytes maturation with immunocytochemical by streptovidin biotin complex method. Oocyteswere maturated treatment by maturation media (TCM-199 that were added FSH 0,01 μg/ml, LH 0,01 μg/ml, BSA 3%) were added by ITS with diffrentiated doses. P0: ITS 0 μg/ml, P1: ITS 10 μg/ml, P2: ITS 15 μg/ml, P3: 20 μg/ml. Oocytes maturation were carried on 38.50C in incubator CO25% for 22 hours. The result showed ITS supplementation in maturation medium P0 (0 μg/ml) has significantly difference (p0,05). The conclusion supplementation ITS on maturation medium be able to increase intracellular calcium expression in oocytes maturation.

Keyword :

Oocytes, IVM, Insulin Transferrin Selenium, calcium intracellular, TCM-199,


References :

Widjiati, D. Wulansari, Wurlina dan NMR. Widjaja.,(2011) Identifikasi Growth Differentiation Factor-9 (GDF-9) Dari Maturasi In Vitro Oosit Sapi dengan Teknik Imunositokimia 5 (2): 59-62. : Jurnal Kedokteran Hewan

Silvestre, F., R. Boni, R.A. Fissore and E.Tosti.,(2011) Ca2+ Signaling During Maturation of Cumulus–Oocyte Complex in Mammals 78:744-756 : Mol. Reprod. Dev

Hasbi, S. Gustina, MA. Setiadi, dan I. Supriatna,(2012) Tingkat Pematangan Inti Oosit Domba dan Pembentukan Pronukleus Setelah Parthenogenesis dengan Penambahan Glutathione 13 (4):445-452 : Jurnal Veteriner





Archive Article

Cover Media Content

Volume : 9 / No. : 1 / Pub. : 2016-02
  1. The Effect Of Refrigerator Temperature And Room Temperature On Total Number Of Bacteria In Meat And Skin Broiler
  2. Isolation And Identification Of Campylobacter Sp Of Raw Chicken Meat Satay Sold By Street Satay Vendors
  3. Supplementation Of Insulin Transferrin Selenium On In Vitro Bovine Oocyte Against To Intracelular Calcium Expression
  4. Potency Of Curcumin On Histopathological Changes In The Liver Of Pregnant Mice Intoxicated By Cadmium
  5. Classical Music Mozart Kv 448 Decrease Abnormality Neuroglia Of Mice (mus Musculus) Hippocampus Exposure To Prenatal Stress
  6. The Potency Of Spirulina Platensis Extract In Reducing The Number Of Polymorpho Nuclear Cells In The Skin Incision Wound Of Mice (mus Musculus)
  7. Reproductive Manipulation Of Java Peafowl (pavo Muticus Muticus)

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