UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 2 / Published : 2011-02

Order : 6, and page :111 - 114

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Original Article :

Efect of centrifugation of goat semen to free radical concentrate on washing medium

Author :

  1. Susilowati, Suherni*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Centrifugation of semen is one of the method for in vitro manipulation of sperm. The aim of centrifugation of semen is to separate motile sperm and immotile sperm and to separate seminal plasm fromsemen that is able to influence quality of sperm. The negative impact of separation of semen by using centrifugation is the production of Reactive Oxygen Species (ROS). ROS has negative effect to sperm metabolism followed by loosing of energy. In this research, semen was centrifugation for 30 minutes and 60 minutes and then evaluated by celuminisence test. The result of this research indicated that concentrate of ROS to show  significantly difference (P<0,05). The conclusion of this research was centrifugation of semen able to cause increasing concentrate of Reactive Oxygen Species (ROS) on sperm.

Keyword :

Semen, Centrifugation, ROS,


References :

Garner and Hafez, E.S.E,(2000) Reproduction in Farm Animal. 7 th Edition. New York : Philadelphia. Baltimore

Dasrul,(2005) Peran Senyawa Oksigen Reaktif Dalam Mekanisme Kerusakan Integritas Membran Spermatozoa Kerbau Lumpur Hasil Sentrifugasi Gradien Densitas percoll Surabaya : Disertasi. Program Pascasarjana. Universitas Airlangga

Agaerwal, A., R.A Saleh and M.A Bedalwy,(2003) Role of Reactive Oxygen Species in the Pathophysiology of Human Reproductive United States : Fertility and Sterility.79:829-849





Archive Article

Cover Media Content

Volume : 4 / No. : 2 / Pub. : 2011-07
  1. The effect of tyrosine kinases addition into the egg yolk skim and egg yolk tris extender the motility and live spermatozoa percentages of the holstein friesian post thawing
  2. Avian influenza h5 virus excretion post challenge test of chickens immunized with avian influenza (h5n1) virus indonesian strain and commercial heterologous vaccine
  3. Reepithelialization and ck 16 expression on superficial-thickness wound with topical hyaluronate and freeze-dried amniotic membrane treatment
  4. The effects of various concentration propanediol cryoprotectan at vitrification process to viability of mice embryos post thawing
  5. Activity test of gynura procumbens leaves extract as antiangiogenic on chick embryo chorioallantoic membrane induced by basic fibroblast growth factor (bfgf)
  6. Efect of centrifugation of goat semen to free radical concentrate on washing medium
  7. Isolation identification and sensitivity test of bacillus subtilis from shrimp culture ponds to antimicrobial agents
  8. The differences in several parameters of the femur bone related to the biomechanics of the bucks and doe
  9. The influence of temperature on meat decay one of beef sold the traditional market
  10. Antibody response and protectivity in chickens after vaccination with nd lv12 active vaccine
  11. Concentration of pregnancy associated glycoprotein (pag) of milk of dairy cows at 21 and 28 days post artificial insemination
  12. The increase of activated goblet cells in the duodenum of broiler exposed by heat stress
  13. (z)-9- heptacosene sex pheromone level production on cuticular hydrocarbon field of strain musca domestica
  14. Antibacterial potency of honey and noni juice (morinda citrifolia l.) to the number of staphylococcus aureus on chicken carcasses
  15. Mengukur kadar protein fertility associated antigen (faa) pada semen sapi menggunakan qubit fluorometer

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