Veterinaria Medika
ISSN 1979-1305
Vol. 10 / No. 2 / Published : 2017-07
Order : 1, and page :131 - 134
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Original Article :
Level of heat shock protein 70 and cortisol in bull’s serum before and after collecting semen
Author :
- Rebecca Mauriella*1
- Pudji Srianto*2
- Budi Utomo*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
Artificial Insemination is a breeding technique by injecting semen into the femalegenital tract by using Artificial Vagina. The process of collecting semen, bulls canexperience of stress which the effect can be affect in reproduction process. This study aimsto prove that the increased levels of HSP 70 and cortisol in bull’s serum after collectingsemen as an indicator of stress in cattle. Blood serum used for ELISA (Enzyme-LinkedImmunosorbent Assay) test using Bovine-HSP 70 Test Kit and Bovine Cortisol Test Kitthen the result read by ELISA reader. The result showed that no significant differences.The conclusion from this study was that elevated levels of HSp 70 and cortisol levelswere not significant, so it is suggested further research on the increased levels of HSP 70and cortisol levels to the manufacture of frozen semen for artificial insemination.
Keyword :
Bull, HSP70, Cortisol, Semen Quality, .,
References :
Hafez, E.S.E.,(2000) Semen Evaluation. In Reproduction in Farm Animals. 7th Ed 363-375 : Lea and Febiger Philadelphia
Hafez, E.S.E.,(2000) Reproduction In Farm Animals. 7Th Ed. 1-12 : Lippincott Wiliams and Wilkins Maryland
Hole J.W.,(1981) Human Anatomy and Phsycology. 2nd. Ed. 213 : Dubuque- Iowa





