UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 2 / No. 1 / Published : 2010-04

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Original Article :

karakterisasi protein lernaea cyprinacea dengan metode elektroforesis sds-page 

Author :

  1. Gunanti Mahasri*1
  2. Ulia Fajriah*2
  3. Sri Subekti *3
  1. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo-Surabaya
  2. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo-Surabaya
  3. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo-Surabaya

Abstract :

One of the diseases that often attack are fish is parasitic disease caused by Lernaea cyprinacea, called Lernaeosis. This ectoparasites could be detected on skin, gill, eyes, fin or inside of mouth and nostril of fishes. The aim of the study was to know the character is tic of L.cyprinacea protein based on molecular weight using SDS-PAGE. The results of this study were expected as Lernaeosis vaccine candidate. The experiment used SDS-PAGE with 12% separating gel, 3% stacking gel, voltage 110 volts 28 A and gel staining using coomassie blue. The result showed that, there were eight protein bands with molecular weight 82.3, 73.3, 66.6, 60.5, 54.9, 27.5, 23.1 and 19.8 kDa. Among them, the bands with molecular weight 27.5, 23.1 and 19.8 kDa were thicker and clearer than the othe rs. 

Keyword :

Lernaeosis, SDS-PAGE, protein ,


References :





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2010-04
  1. Technology of daphnia sp culture ”daily feeding” using rice bran solution to water quality and daphnia sp population
  2. Identification of bacterial fish pathogen and their number from shrimp and rearing water
  3. Sediment quality analysis on intensif pond and their relationship with tiger prawn (penaeus monodon fab.)production
  4. The effect of commercial feeds on growth and color changes of red diamond lou han
  5. Teknik budidaya rumput laut (gracilaria verrucosa) dengan metode rawai di balai budidaya air payau situbondo jawa timur
  6. Pengaruh lama penyinaran terhadap pertumbuhan dan klorofil a gracilaria verrucosa pada sistem budidaya indoor
  7. korelasi antara konsentrasi oksigen terlarut pada kepadatan yang berbeda dengan skoring warna daphnia spp. 
  8. kandungan protein kasar dan serat kasar pada daun kangkung air (ipomoea aquatica) yang difermentasi 
  9. manajemen pembesaran kerapu tikus (cromileptes altivelis) di balai besar pengembangan budidaya air payau (bbpbap) jepara jawa tengah 
  10. karakterisasi protein lernaea cyprinacea dengan metode elektroforesis sds-page 
  11. pengaruh pemberian vaksin whole cell killed virus terhadap sintasan udang vannamei (lithopenaeus vannamei) yang diinfeksi whitespot baculovirus (wsbv) 
  12. teknik pemeliharaan benih rajungan (portunus pelagicus linn.) di balai besar pengembangan budidaya air payau jepara kabupaten jepara propinsi jawa tengah 
  13. teknik penanganan induk ikan koi (cyprinus carpio l.) di kelompok usaha tani sumber harapan desa kemloko kecamatan nglegok kabupaten blitar propinsi jawa timur 
  14. Jellyfish sting : an indonesian case report 
  15. daya antibakteri ekstrak buah adas (foeniculum vulgare) terhadap bakteri micrococcus luteus secara in vitro 
  16. pengaruh persentase pertukaran air pada pertumbuhan gracilaria verrucosa dalam budidaya bak terkontrol 
  17. populasi dan keanekaragaman hewan makrobenthos pada perairan tertutup dan terbuka di teluk awur, jepara 

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