UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 23 / No. 3 / Published : 2007-09

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Original Article :

Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique

Author :

  1. M. Gandul Atik Yuliani*1
  2. Jola Rahmahani*2
  3. Suwarno*3
  1. Bagian Ilmu Kedokteran Dasar Laboratorium Patologi Klinik , Fakultas Kedokteran Hewan Universitas Airlangga Kampus C, Mulyorejo, Surabaya 60115, Telp(031)5992785 Fax(031)5993015
  2. Bagian Mikrobiologi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga Kampus C, Mulyorejo, Surabaya 60115, Telp(031)5992785 Fax(031)5993015
  3. Bagian Mikrobiologi Veteriner
    Fakultas Kedokteran Hewan Universitas Airlangga
    Kampus C, Mulyorejo, Surabaya 60115, Telp(031)5992785 Fax(031)5993015

Abstract :

The aim of this research is to know the using of monoclonal antibody from G protein of rabies virus that used in ELISA test. In the research to measure monoclonal antibody reactivity by thinning the monoclonal antibody from 10-1 to 10-10. To evaluate samples from suspect animal is used two kind sample, saliva and brain suspension from mice had be injected field strain rabies virus isolate. Afterwards samples were evaluated by indirect double antibody sandwich ELISA. The result showed that monoclonal antibody G protein can detection rabies virus from saliva and brain. The conclusion of the riset is showed that monoclonal antibody from G protein of rabies virus reactively for detection rabies virus.

Keyword :

Antibody from G Protein, rabies Virus, ELISA Technique,


References :

Center of Dissease Control,(2003) Rabies - : http: //www.cdc.gov/ncidod/ dvrd

L Charles,(2001) Rabies in American bison from North Dakota - : J. Wildlife Disease. 96(1)

M Fekadu,(1997) An immune stimulating complex (ISCOM) sub unit rabies vaccine protects dog and mice againts street rabies challange - : J. Vaccine 10(3)

RA Goldsby,(2000) Kuby Immunology. Fourth Edition New York : WH. Freeman and Company

W Ikematsu,(1998) Clonal analysis of human antibody respon. III. Nucleotide sequences of monoclonal IgM, IgGand IgA to rabies virus reveal restricted V gene utilization. Junctional V J and VJ diversity and somatic hypermutation - : J. Immunology. 161

N Ito,(2001) Rescue of rabies virus from cloned cDNA and identification of the pathogenecity-related gene: glycoprotein gene Is associated with virulence for adult mice - : J. Virology. 19(75)

S Modrow,(1997) Rhabdoviren. Moleculare Virology Heidelberg. Berlin : Spectrum Academicher V



Archive Article

Cover Media Content

Volume : 23 / No. : 3 / Pub. : 2007-03
  1. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens
  2. Protein characterization of snail mucin (achasin) local isolate as an antibacterial factor
  3. Trypsin and pronase treatment on the development of mouse embryos contaminated by k99 escherichia coli
  4. The optimation of tyrosine kinase activity from sperm isolation of friesian holstein cow
  5. Insulin-like growth factor i (igf-i) complex seminal plasma of goatratio glicoprotein : protein : carbohydrate
  6. The role of choline esterase on vesicle brain development of chicken embryo of exposure by carbofuran insecticide
  7. Marker-sets for paternity and identity test in pigs by meansmicrosatellite analysis
  8. Bcg vaccination increased roi secretion in dogs macrophage infected with mycobacterium tuberculosis
  9. Antifertility effect of aqoueus extract betel nut of areca catechu: as an apoptosis agent on testis connective cells of rattus norvegicus
  10. The morphology of mandibular and lingual glands of chicken (gallus sp) and quail (coturnix coturnix): with special refference to the distributionand carbohydrate content
  11. Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique
  12. Study to rice brand in drying ensilage product from edible snail for decrease higroscopies as feed stuff
  13. Potency rice straw was deammoniated and fermented by celulolytic bacteria on the dry matter consumption, body weight gain and feed conversion of sheep
  14. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens
  15. Protein characterization of snail mucin (achasin) local isolate as an antibacterial factor
  16. The role of choline esterase on vesicle brain development of chicken embryo of exposure by carbofuran insecticide
  17. The optimation of tyrosine kinase activity from sperm isolation of friesian holstein cow
  18. Insulin-like growth factor i (igf-i) complex seminal plasma of goat ratio glicoprotein : protein : carbohydrate
  19. Marker-sets for paternity and identity test in pigs by means microsatellite analysis
  20. Bcg vaccination increased roi secretion in dogs macrophage infected with mycobacterium tuberculosis
  21. Antifertility effect of aqoueus extract betel nut of areca catechu: as an apoptosis agent on testis connective cells of rattus norvegicus
  22. Trypsin and pronase treatment on the development of mouse embryos contaminated by k99 escherichia coli
  23. The morphology of mandibular and lingual glands of chicken (gallus sp) and quail (coturnix coturnix): with special refference to the distribution and carbohydrate content
  24. Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique
  25. Study to rice brand in drying ensilage product from edible snail for decrease higroscopies as feed stuff
  26. Potency rice straw was deammoniated and fermented by celulolytic bacteria on the dry matter consumption, body weight gain and feed conversion of sheep
  27. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens

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