UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 21 / No. 2 / Published : 2015-03

Order : 17, and page :191 - 195

Related with : Scholar   Yahoo!   Bing

Original Article :

Storage temperature for 24 hours of uric acid in urine

Author :

  1. AAN. Subawa*1
  2. Sianny Herawati*2
  3. I Nyoman Wande*3
  4. I Wayan Putu Sutirta Yasa*4
  5. Tjokorda Gede Oka*5
  1. Bagian Patologi Klinik FK Universitas Udayana/RSUP Sanglah Denpasar
  2. Bagian Patologi Klinik FK Universitas Udayana/RSUP Sanglah Denpasar
  3. Bagian Patologi Klinik FK Universitas Udayana/RSUP Sanglah Denpasar
  4. Bagian Patologi Klinik FK Universitas Udayana/RSUP Sanglah Denpasar
  5. Bagian Patologi Klinik FK Universitas Udayana/RSUP Sanglah Denpasar

Abstract :

Creatinine and uric acid is a product that excreted in the urine by normal kidney functions. The examination of creatinine and uric acid in urine is done on 24-hour urine collection. During the storage of the urine, it is recommended to be stored in a refrigerator with the grade temperatures ranging from 2–8°C and is not recommended to use any preservative for the examination of creatinine and uric acid in urine. To know the comparation of creatinine and uric acid concentrations in urine between the urine tested immediately after the collection with urine that was stored at a temperature 2–8°C and those at room temperature for 24 hours. A total of 45 urine samples from outpatient clinic that came to the laboratory, were collected in particular urine vacutainer. Each urine sample is divided into three tubes. The first tube (P1) examined concentrations of creatinine and uric acid immediately after collection, was considered as the baseline value. The second tube (P2) stored at 2–8°C and the third tube (P3) is stored at room temperature for 24 hours, then followed by the examination of creatinine and uric acid concentrations. The examination of creatinine in urine was using reagent CREP2 Roche Diagnostic and uric acid in urine was using reagent UA2 Roche diagnostics by Cobas Integra ® 400 plus ® instrument. The mean of creatinine in urine concentrations which immediately examined (P1) is (125.10±74.85 mg/dL), concentrations after storage at 2−8°C (P2) and at room temperature (P3) were (123.42±73.80 mg/dL) and (124.09±73.95 mg/dL) respectively. Based on the analysis of one-way ANOVA, there were no significant differences between the concentrations of creatinine in urine immediately checked which were stored at 2–8°C and at room temperature (P>0.05). The mean of uric acid in urine concentrations which immediately examined (P1) is (52.61±35.48 mg/dL), where as after storage at 2–8°C (P2) and room temperature (P3) were (45.11±31.62 mg/dL) and (46.38±28.91 mg/dL) respectively. Based on the analysis of one-way ANOVA, there were no significant differences between the concentrations of uric acid in urine immediately checked by those stored at 2–8°C and at room temperature (P>0.05). Based on this study, it can be concluded that there were no effect of storage temperature on the concentrations of creatinine and uric acid in urine within 24 hours. Kreatinin dan asam urat merupakan produk tertentu diekskresikan dalam air kemih yang diperankan oleh fungsi ginjal yang normal. Pemeriksaan kreatinin dan asam urat dalam air kemih dilakukan dengan menampung air kemih selama 24 jam. Selama penampungan air kemih tersebut dianjurkan menyimpannya dalam lemari pendingin dengan suhu berkisar antara 2−8°C dan pada pemeriksaan kreatinin dan asam urat dalam air kemih jangan (tidak dianjurkan) menggunakan pengawet. Tujuan kajian ini untuk mengetahui kadar kreatinin dan asam urat dalam air kemih dengan membandingkan antara air kemih yang segera diperiksa dan yang disimpan pada suhu antara 2−8°C dan yang suhu kamar. Sebanyak 45 sampel air kemih yang berasal dari pasien rawat jalan yang datang ke laboratorium klinik di tampung di dalam wadah kedap udara khusus untuk itu. Setiap sampel air kemih kemudian dibagi ke dalam tiga tabung. Tabung pertama (P1) diperiksa kadar asam urat segera setelah penampungan, dianggap sebagai nilai garis dasar. Tabung kedua (P2) disimpan pada suhu 2−8°C dan tabung ketiga (P3) disimpan pada suhu kamar selama 24 jam, kemudian kadar kreatinin dan asam urat diperiksa. Pemeriksaan kreatinin air kemih menggunakan reagen CREP2 Roche Diagnostic dan asam urat air kemih menggunakan reagen UA2 Roche diagnostic dengan alat cobas Integra 400 plus®. Rerata kadar kreatinin air kemih yang segera diperiksa (P1) yaitu 125,10±74,85 mg/dL, kadar setelah disimpan pada suhu antara 2−8°C (P2) yaitu 123±73,80 mg/dL dan setelah disimpan pada suhu kamar (P2) yaitu 124,09±73,95 mg/dL. Berdasarkan analisis dengan One-Way ANOVA, tidak terdapat perbedaan bermakna kadar kreatinin air kemih antara ketiga kelompok (p>0,05) Rerata kadar asam urat air kemih yang segera diperiksa (P1) yaitu (52,61±35,48 mg/dL), kadar setelah disimpan pada suhu 2−8°C (P2) yaitu (45,11±31,62 mg/dL) dan rerata kadar setelah disimpan pada suhu kamar yaitu (46,38±28,91 mg/dL). Berdasarkan analisis One Way ANOVA, tidak terdapat perbedaan bermakna kadar asam urat antara air kemih yang segera diperiksa dengan yang disimpan pada suhu antara 2−8°C dan yang suhu kamar (p>0,05). Berdasarkan telitian ini ternyata suhu pada pemeriksaan kadar kreatinin dan asam urat air kemih yang disimpan selama 24 jam tidak berpengaruh

Keyword :

Creatinine urine, uric acid urine, storage temperature, 24 hours,


References :

Fischbach F,(2000) Urine study: A Manual of Laboratory & Diagnostic Test. Sixth Ed 170–279 : Philadelphia, Lippicott

Cole Y. Uric,(2005) acid in urine: A-Z Health Guide from WebMD: Medical Tests. 1–5 : WebMD Health Center





Archive Article

Cover Media Content

Volume : 21 / No. : 2 / Pub. : 2015-03
  1. Pneumatic Tube On Routine Blood Test And Lactate Dehydrogenase
  2. Detection In Tetrazolium Microplate Assay Culture Methods From Pulmonary Tuberculosis Suspected Sputum
  3. High Molecular Weight Adiponectin And Vasculer Thickness In Diabetes Type 2 Related To Fixed Dose Combination Of Glimepiride And Metformin
  4. Neutrofil/lymphocyte Ratio In Carcinoma Mammae
  5. Platelet Aggregation And Mean Volume With Metabolic Syndrome In Obesity
  6. Diagnosis Of Pulmonary Tuberculosis Based On Frequency Of Sputum Examination
  7. Genetic Variation Of Hepatitis B Virus Polymerase Gene From Chronic Hepatitis B Infected Patient With Telbivudine Therapy
  8. The 38 Kda Adhesin Protein Of Mycobacterium Tuberculosis And Macrophage Of The Lung
  9. Bacterial Pattern And Patient’s Age On Procalcitonin In Community And Hospital Acquired Pneumonia
  10. Aspergillus Glaucus Group And Penicillium Sp In Neurosurgery Operating Theater
  11. Diagnostic Value Of Iga Antivca Epstein-barr Antibody In Nasophryngeal Carcinoma
  12. Blood Glucose Test Between Hexokinase With Glucose Oxidase And Glucose Dehydrogenase Methods In Diabetes Mellitus
  13. β-thalassemia Trait Using Capillary Electrophoresis
  14. Lipoprotein(a) In Acute Coronary Syndrome
  15. D-dimer Plasma Levels In Ishemic Stroke
  16. Adrenomedullin’s In Breast Cancer With Metastatic State
  17. Storage Temperature For 24 Hours Of Uric Acid In Urine
  18. Ebola Virus Disease
  19. Congenital Malaria

Information CPML