Media Kedokteran Hewan
ISSN 2015-8930
Vol. 23 / No. 3 / Published : 2007-08
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Original Article :
Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique
Author :
- M. Gandul Atik Yuliani*1
- Jola Rahmahani*2
- Suwarno*3
- Bagian Ilmu Kedokteran Dasar Laboratorium Patologi Klinik ,Fakultas Kedokteran Hewan Universitas Airlangga
Kampus C, Mulyorejo, Surabaya 60115, Telp(031)5992785 Fax(031)5993015 - Bagian Mikrobiologi Veteriner,Fakultas Kedokteran Hewan Universitas Airlangga
Kampus C, Mulyorejo, Surabaya 60115, Telp(031)5992785 Fax(031)5993015 - Bagian Mikrobiologi Veteriner,Fakultas Kedokteran Hewan Universitas Airlangga Kampus C, Mulyorejo, Surabaya 60115, Telp(031)5992785 Fax(031)5993015
Abstract :
The aim of this research is to know the using of monoclonal antibody from G protein of rabies virus that used in ELISA test. In the research to measure monoclonal antibody reactivity by thinning the monoclonal antibody from 10-1 to 10-10. To evaluate samples from suspect animal is used two kind sample, saliva and brain suspension from mice had be injected field strain rabies virus isolate. Afterwards samples were evaluated by indirect double antibody sandwich ELISA. The result showed that monoclonal antibody G protein can detection rabies virus from saliva and brain. The conclusion of the riset is showed that monoclonal antibody from G protein of rabies virus reactively for detection rabies virus.
Keyword :
Antibody from G Protein, rabies Virus, ELISA Technique ,
References :
J Rahmahani,(2002) Karakterisasi Glikoprotein Virus Rabies Strain Alam Pada Pembuatan Antibodi Monoklonal Rabies Surabaya : Universitas Airlangga
Suwarno,(2003) Produksi Antibodi Monoklonal Dengan Antigen Spesifik untuk Tujuan Imuno¬diagnostik dan Imunoterapi Surabaya : Universitas Airlangga





