Veterinaria Medika
ISSN 1979-1305
Vol. 1 / No. 1 / Published : 2008-02
Order : 6, and page :29 - 32
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Original Article :
Antibacterial activity of the ethanol extract pluchea indica less leaves against escherichia coli by in vitro
Author :
- Ari Susanti*1
- Rimayanti*2
- Muhammad Sukmanadi*3
- PPDH Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
The aim of this study was to investigate the antibacterial activity of the ethanol extract Pluchea indica lessleaves against Escherichia coli by in vitro. The method using broth dilution test was determined MinimumBactericidal Concentration (MBC) with a broth extract cultures into Eosin Methylen Blue Agar (EMBA)medium. The twice tubes of concentration extract of 25% and 50% was showed no visible turbidity, and theninoculated into Eosin Methylen Blue Agar medium. After 24 h of incubation at 37 oC, Minimum BactericidalConcentration (MBC) was determined which no viable growth of Escherichia coli at Eosin Methylen BlueAgar (EMBA) medium. The result showed that Minimum Bactericidal Concentration (MBC) of the extract of25% and 50% had been similar (X2<0.05) on the growth of Escherichia coli. Based on this result, MinimumBactericidal Concentration (MBC) of the extract was used 25% concentration.
Keyword :
antibacterial, Pluchea indica less, Escherichia coli, ., .,
References :
Hariana, A.,(2006) Tumbuhan Obat dan Khasiatnya. Seri 1 Jakarta : Penebar Swadaya
Anto,(2003) Penggunaan Antibiotika Dalam Budidaya Unggas Jakarta : Poultry Indonesia





