UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 39 / No. 1 / Published : 2003-01

Order : 3, and page :3 - 9

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Original Article :

Population data on the locus d16s83 of vntr (variable number of tandem repeat) polymorphism in the population around surabaya, east java, indonesia

Author :

  1. Soekry Erfan Kusuma*1
  1. Department of Forensic Medicine Airlangga University School of Medicine Dr Soetomo Teaching Hospital, Surabaya

Abstract :

To calculate the probability value of the real father in a paternity test, frequency distribution of every alleles and genotype of polymorphism loci is needed. However, data on that distribution in Surabaya, as well as in Indonesia, are not available. In providing such data, we studied 59 unrelated population of Surabaya . Locus D16S83 of Variable Number of Tandem Repeat (VNTR) was chosen in this study, in which the DNA samples were extracted from blood cells. The samples were subjected to PCR and, subsequently, to polyacrylamide agarose composite gel electrophoresis and silver staining. From 12 alleles of D16S83 taken from 59 individuals in Surabaya, only alleles number 1, 2, 3, 4, 5, 6 ,9, 10, 11, and 12 were found. The frequency of allele 1 was 36.4%, allele 2 was 21.1%, allele 3 was 10.1% and the others were less than 9.32%. The frequency of heterozygote was 74.5 %, phenotype 1-2 was 32.2%, phenotype 1-3 was 10.1%, phenotype 1-4 was also 10.1 % and the others were less than 8.4%. A comparison of the results of this study was also made to other studies performed by other authors on the Caucasians and Afro-Carribeans

Keyword :

D16S83 locus, polyacrylamide agarose composite gel, PCR, allele distribution frequency,


References :

Rangel-Villalobus H, et al.,(1999) Allele frequency distributions of six Amp-FLPS (D1S80, APO-B, VWA, THO1, CSF1PO and HPRTB) in a Mexican population - : Forensic Sci Int

Deka R, Susan DeCroo, Ling Mei Yu, and Robert E Ferrel,(1992) Variable number of tandem repeat (VNTR) polymorphism at Locus D17S5 (YNZ 22) in four ethnically defined human population - : Hum Genet





Archive Article

Cover Media Content

Volume : 39 / No. : 1 / Pub. : 2003-01
  1. Editorial Vol 39 No 1 2003
  2. Opinion: Problem Based Learning And The Change Of Academic Behaviour
  3. Population Data On The Locus D16s83 Of Vntr (variable Number Of Tandem Repeat) Polymorphism In The Population Around Surabaya, East Java, Indonesia
  4. An Immunohistochemical Analysis Of Tgf-ß1, As A Predictor Of Delayed Wound Healing In Episiotomy Surgery Of Sprague Dawley Rats
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