UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 23 / No. 3 / Published : 2007-09

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Original Article :

Marker-sets for paternity and identity test in pigs by meansmicrosatellite analysis

Author :

  1. Siti Darodjah Rasad*1
  1. Laboratorium Reproduksi Ternak, Fakultas Peternakan UNPAD
    Kampus Jatinangor, Bandung 40600. Tel. 022-7798341, Fax. 022-7798212

Abstract :

The aim of this study was to establish an optimal marker set for a routine paternity and identity test. In the present work a paternity and identity test within three pig breeds (DE, DL and Pi) was performed by means of microsatellite analysis. Genotyping of the animals was done after PCR amplification of the alleles on an automated DNA sequencer (LI-COR, DNA Analyzer, GENE READIR 4200 (MWG BIOTECH). For the examination of the applicability of the microsatellite markers in paternity test, the exclusion probabilities and Polymorphism Information Contents (PICs) were calculated. Combined exclusion probability over 99 % has been achieved in all breeds by using 8 microsatellites. Fifteen of the 25 loci were selected and a group of 5 loci were packed together in three multiplex-PCRs as possible marker sets for a paternity and identity test in pig.

Keyword :

Pig, Paternity and Identity Test, Microsatellite analysis,


References :

RB Alford,(1994) Rapid and efficient resolution of parentage by amplification of short tandem repeats - : Am J Hum Genet. 55

I Alvarez,(2004) Genetic relationships and admixture among sheep breeds from Northern Spain assessed using microsatellites - : Journal of Animal Science. 82

JJ Arranz,(2001) Differentiation among Spanish sheep breeds using microsatellites - : Genetics Selection Evolution. 33

JC Avise,(1994) Molecular Markers. Natural History and Evolution New York : Chapman and Hall. Inc

R Baumung,(2004) Genetic diversity studies in farm animals – a survey - : Journal of Animal Breeding and Genetics. 121

MM Binns,(1995) The identification of polymorphic microsatellite loci in the horse and heir use in thoroughbred parentage testing - : British Vet J. 151

D Botstein,(1980) Construction of a genetic linkage map using restriction fragment length polymorphism - : Am J Hum Genet. 32

AT Bowling,(1997) Validation of



Archive Article

Cover Media Content

Volume : 23 / No. : 3 / Pub. : 2007-03
  1. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens
  2. Protein characterization of snail mucin (achasin) local isolate as an antibacterial factor
  3. Trypsin and pronase treatment on the development of mouse embryos contaminated by k99 escherichia coli
  4. The optimation of tyrosine kinase activity from sperm isolation of friesian holstein cow
  5. Insulin-like growth factor i (igf-i) complex seminal plasma of goatratio glicoprotein : protein : carbohydrate
  6. The role of choline esterase on vesicle brain development of chicken embryo of exposure by carbofuran insecticide
  7. Marker-sets for paternity and identity test in pigs by meansmicrosatellite analysis
  8. Bcg vaccination increased roi secretion in dogs macrophage infected with mycobacterium tuberculosis
  9. Antifertility effect of aqoueus extract betel nut of areca catechu: as an apoptosis agent on testis connective cells of rattus norvegicus
  10. The morphology of mandibular and lingual glands of chicken (gallus sp) and quail (coturnix coturnix): with special refference to the distributionand carbohydrate content
  11. Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique
  12. Study to rice brand in drying ensilage product from edible snail for decrease higroscopies as feed stuff
  13. Potency rice straw was deammoniated and fermented by celulolytic bacteria on the dry matter consumption, body weight gain and feed conversion of sheep
  14. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens
  15. Protein characterization of snail mucin (achasin) local isolate as an antibacterial factor
  16. The role of choline esterase on vesicle brain development of chicken embryo of exposure by carbofuran insecticide
  17. The optimation of tyrosine kinase activity from sperm isolation of friesian holstein cow
  18. Insulin-like growth factor i (igf-i) complex seminal plasma of goat ratio glicoprotein : protein : carbohydrate
  19. Marker-sets for paternity and identity test in pigs by means microsatellite analysis
  20. Bcg vaccination increased roi secretion in dogs macrophage infected with mycobacterium tuberculosis
  21. Antifertility effect of aqoueus extract betel nut of areca catechu: as an apoptosis agent on testis connective cells of rattus norvegicus
  22. Trypsin and pronase treatment on the development of mouse embryos contaminated by k99 escherichia coli
  23. The morphology of mandibular and lingual glands of chicken (gallus sp) and quail (coturnix coturnix): with special refference to the distribution and carbohydrate content
  24. Detection of rabies virus in saliva and brain using antibody from g protein as material diagnostic by indirect double antibody sandwich elisa technique
  25. Study to rice brand in drying ensilage product from edible snail for decrease higroscopies as feed stuff
  26. Potency rice straw was deammoniated and fermented by celulolytic bacteria on the dry matter consumption, body weight gain and feed conversion of sheep
  27. Cecal goblet cell response on intracellular development of eimeria tenella in susceptible and infected chickens

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