Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

Order : 1, and page :68 - 73

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Original Article :

Immunogenity of 32.2 kda hemagglutinin bm protein of escherichia coli pili isolated from infertile males' semen

Author :

  1. Sukarjati*1
  2. Doddy M Soebadi*2
  3. Aucky Hinting*3
  4. Sudjarwo*4
  1. Department of Biology, PGRI Adi Buana University, Surabaya
  2. Department of Urology, Airlangga University Faculty of Medicine, Surabaya
  3. Department of Medical Biology, Airlangga University Faculty of Medicine, Surabaya
  4. Department of Pharmacochemistry, Airlangga University Faculty of Pharmacy, Surabaya

Abstract :

Male genital tract infection is one cause of infertility. Escherichia coli (E. coli) is a major cause of prostatitis and epididymitis that can degrade the quality of spermatozoa. One factor E. coli can reduce sperm quality due to the ability of E. coli attached to the sperm plasma membrane using pili. Have successfully isolated the protein hemagglutinin pili of E. coli isolates were infertile men with BM cement 32.2 kDa. The purpose of this study was to determine the protein hemagglutinin pili immunogenitas E. BM coli isolates 32.2 kDa cement infertile men. Immunization performed on male rabbits using protein hemagglutinin pili of E. coli BM 32.2 kDa as antigens. Antibody titers formed were measured using indirect methods and the results were read using elisa reader. Antigen and antibody reactivity are formed in the test by the method of dot blot and western blot. The results of this research is through indirect elisa test, the protein hemagglutinin pili of E. BM coli isolates 32.2 kDa cement infertile men can stimulate the formation of antibodies. The results of dot blot test and western blot proved that the antibodies react specifically with antigen. The conclusion of this study is the protein hemagglutinin pili of E. coli BM 32.2 kDa is immunogenic and potentially as a candidate vaccine ingredient in preventing male genital tract infections.

Keyword :

protein hemagglutinin pili of E. coli BM 32.2 kDa, immunogenity, indirect elisa, western blot, dot blot,

References :

Aronson,M.O., Medalia O., Schori, L., Mirelman, D., Sharon, N., Ofek, I., ,(1979) Prevention of colonization of the urinary tract of mice with E. coli by blocking of bacterial adherence with methyl-alpha-mannopyranoside - : J.Infect.Dis.

Connell,H., Agace,W., Klemm, P., Schembri, M., Marild,S., Svanborg,C.,(1996) Type 1 fimbrial expression enhances E. coli virulence for the urinary tract - : Proc.Natl. Acad.Sci

Cummins JM.,(1995) Tests of Sperm Function Cambridge : Cambridge University Press

Diemer T., Weidner W., Michelmann HW., Schiver HG., Rovan E., and Mayer F.,(1996) Influence of E. coli on motility parameters of human spermatozoa in vitro - : Int. J. of Andrology

Henahan S.,(1997) E. coli Vaccine - : Science Update

Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
  1. Immunogenity Of 32.2 Kda Hemagglutinin Bm Protein Of Escherichia Coli Pili Isolated From Infertile Males' Semen
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